84-4443-67　PICK1, CT (PRKCA-binding Protein, Protein Interacting with C Kinase 1, Protein Kinase C-alpha-binding Protein) 100ul　366981

特徴

• PRKCA-binding protein (UniProt Q62083; also known as Protein interacting with C kinase 1, Protein kinase C-alpha-binding protein) is encoded by the Pick1 (also known as Prkcabp) gene (Gene ID 18693) in murine species.
• PICK1 is a scaffolding protein expressed in many tissues, where it regulates trafficking of multiple membrane proteins.
• The PDZ (PSD-95/Dlg/ZO-1/DHR (Dlg homologous region) domain (aa22-105) mediates PICK1 interaction with proteins containing PDZ-binding motifs, such as PKC and acid sensing ion channels (ASICs), while the lipid-binding AH (arfaptin homology)/BAR (Bin/amphiphysin/Rvs) domain (aa144-357) mediates PICK1 homo-dimerization and membrane localization.
• Pick1 gene-disruption results in downregulated ASICs current and calcium transients during process of ischemia-reperfusion and protects against primary murine cortical neurons against injury caused by OGD-Rep (oxygen and glucose deprivation followed by reperfusion).
• PICK1 is also shown to act as a negatively regulator against ASIC1 phosphorylation/activation by PKA in rat pulmonary arterial smooth muscle cells, where PICK1 facilitates calcineurin-mediated ASIC1 dephosphorylation.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry (Paraffin): 1:1000 detects PICK1 in human cerebellum, kidney, and pancreas tissue sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.