84-4443-65　Oxytocin Receptor, NT (OT-R, OXTR) 100ul　366977

特徴

• Oxytocin receptor (UniProt P97926; also known as OT-R) is encoded by the Oxtr (also known as Otr) gene (Gene ID 18430) in murine species.
• Oxytocin receptor (OT-R) is widely expressed in the reproductive tract, brain, kidneys, and many other organs, where it mediates the biological functions of oxytocin (OT) in mammals.
• Best known for their role in lactation and parturition, OT-R and OT meidate a wide variety of physiological functions, including estrous cycle, bone formation, social behaviors, as well as regulation of energy homeostasis, including food intake and thermoregulation.
• Central administration of OT decreases food consumption in rats, and nesfatin (a satiety hormone) increases OT secretion.
• Oxt-null and Oxtr-null mice display aberrant thermoregulatory function.
• In addition, Oxtr-knockout mice have a morphological defect in brown adipose tissue (BAT) cells and develop obesity.
• Human and murine oxytocin receptors are 7-transmembrane (aa39-63, 75-97, 114-135, 155-175, 203-225, 275-293, 309-331) GPCRs with their N-terminal end at the extracellular side (aa1-38) and C-terminal end at the cytoplasmic side (human aa332-389 and mouse aa332-388).
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500 detects oxytocin receptor in 10ug of A431, HEK293, HeLa, Jurkat, MCF-7, and Raji membrane extractsImmunofluorescence: 1:1000 detects oxytocin receptor immunoreactivity in wild-type, but not Oxtr-knockout, mouse frozen brain tissue sectionsOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.