Substance-P receptor (UniProt P25103; also known as Neurokinin-1 receptor, NK-1 receptor, NK-1R, SPR, Tachykinin receptor 1) is encoded by the TACR1 (also known as NK1R, TAC1R) gene (Gene ID 6879) in human.
NK-1, NK-2 and NK-3 are receptors that mediate the biological activities of tachykinins, including substance P (SP), neurokinin A (NKA), neurokinin B (NKB), ranakinin, kassinin, neuropeptide gamma, and eledoisin, widely distributed in the central and peripheral nervous systems.
SP shows the highest affinity toward NK-1R (substance-P receptor) and exerts its biological activities via NK-1R, including addiction, depression, chronic inflammation, and cancer progression.
The involvement of NK-1R in acute pancreatitis through mediating neurogenic inflammation has also been reported.
Upon stimulation by SP, NK-1R interacts with multiple G proteins, including G s, G q/11, G i/o, G 12, and G 13, generating second messengers and activating downstream effector molecules.
After binding NK-1R, SP induces a clathrin-dependent internalization of the receptor-ligand complex, SP is then degraded and the receptor is recycled to the cell surface.
NK-1R is a 7-transmembrane (32-54, 65-86, 107-128, 149-169, 195-219, 249-270, 284-308) GPCR, having its N-terminal end (aa1-31) at the extracelluar side and C-terminal end (aa309-407) at the cytoplasmic side.
There exists a second alternatively spliced human NK-1R isoform that lacks the C-terminal cytoplasmic domain (aa312-407 missing).
The full-length form enhances the growth of cancer cells and stimulates the production of cytokines, which in turn up-regulate the truncated form.
The truncated form mediates malignancy and is more abundant in colonic epithelial cells from patients with colitis-associated cancer.
In cancer cells, the NK-1 receptor is mainly localized on the plasma membrane and in cytoplasm, and occasionally also in the nucleus.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:50 detects substance P receptor in human, mouse, and rat brain tissue sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes human substance P receptor C-terminal. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：KLH-conjugated, linear peptide corresponding to the C-terminal end sequence of human substance P receptor. Species sequence homology (100%): rhesus macaque, canine
