84-4443-53　Cullin-4A, NT (CUL-4A) 100ul　366945

特徴

• Cullin-4A (UniProt Q13619; also known as CUL-4A) is encoded by the CUL4A gene (Gene ID 8451) in human.
• CUL-4A belongs to the cullin family of hydrophobic proteins that functions as the scaffold component of E3 ubiquitin ligase complexes.
• CUL-4A interacts with DNA damage binding protein 1 (DDB1) and ring of cullins (ROC1) to assemble the E3 complex, where DDB1 acts a linker protein for CUL4 and recruits substrates to the CUL4-ROC1 catalytic core through interaction with a subset of WD40 proteins.
• At least 90 DDB1-binding WD40 (DWD) proteins (also known as DCAF for Ddb1- and Cul4-associated factors and CDW for CUL4 and DDB1-associated WD40 repeats) are known, suggesting a large number of potential substrates for CUL4-ROC1 ligase complexes.
• CUL4-ROC1 E3 ligase complexes play important roles in cell cycle, DNA replication, genomic stability, haematopoiesis, and spermatogenesis.
• CUL-4A is highly expressed in testis and spleen, while poor expression is found in lung, liver, thymus, small intestine, and muscular tissues.
• CUL4A gene amplification has been demonstrated in many kinds of tumors, including hepatocellular carcinomas (HCC), squamous cell carcinomas, and adrenocortical carcinoma.
• CUL4A silencing is reported to suppress the motility of HCC cells and reverse their epithelial-mesenchymal transition (EMT) tendency.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500 detects Cullin-4A in 10 ug of HeLa cell lysate
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.