Bromodomain-containing protein 4 (UniProt O60885; also known as MCAP, Mitotic chromosome-associated protein, Protein HUNK1) is encoded by the BRD4 (also known as HUNK1, MCAP) gene (Gene ID 23476) in human.
BRD4 is a BET (bromodomain and extra-terminal) protein family member that functions as a chromatin reader by binding acetylated lysines in histones.
BRD4 is an important transcription regulator in many cell types, including transcription in response to external cues.
BRD4 plays an essential role in neuronal function and mediates the transcriptional regulation underlying learning and memory.
BRD4 regulates IEG transcription in neurons upon phosphorylation by casein kinase 2 (CK2), which is activated in response to neuronal stimulation.
Brd4 knockout in mice is lethal, while Brd4 function blockage by inhibitor JQ1 treatment is reported to affect the transcription of critical synaptic proteins, resulting in memory deficits and decreased seizure susceptibility in mice.
Applications:Suitable for use in Western Blot, Dot Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Immunocytochemistry: 1:500 detects nuclear Brd4 pSer493Western Blot: 1:500 detects Brd4 Ser493 (equivalent to human Ser492) phosphorylation in lysate from 12-day cultured E16.5 embryonic mouse cortical neurons.
Prior phosphatase treatment of the lysate abolished target band detectionOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes human BRD4. Species Crossactivity: mouse. The target phospho-serine residue is present in all human (pSer492) and murine (pSer493) BRD4 spliced isoforms reported by UniProt (O60885 and Q9ESU6). 366930 is shown to exhibit stronger immunoreactivity toward BRD4 phosphopeptide with dual pSer492/494 (equivalent to mouse pSer493/495) phosphorylation than the corresponding peptide with only pSer492 (equivalent to mouse Ser493) phosphorylation.
Isotype：IgG
Calc Applications Abbrev：DB IC WB
Calc Crossreactivity：Hu Mo
Immunogen：KLH-conjugated linear peptide corresponding to a human BRD4 sequence containing phosphorylated Ser492 (equivalent to mouse Ser493). Species Sequence Homology (100%): rat
