84-4443-49　［取扱停止］BPTF (Nucleosome-remodeling Factor Subunit BPTF, Bromodomain and PHD Finger-containing Transcription Factor, Fetal Alz-50 clone 1 Protein, Fetal Alzheimer Antigen) [KO Validated] 100ul　366928

特徴

• Nucleosome-remodeling factor subunit BPTF (UniProt A2A654 and E9Q6A7; also known as Bromodomain and PHD finger-containing transcription factor, Fetal Alz-50 clone 1 protein, Fetal Alzheimer antigen) is encoded by the Bptf (also known as Fac1, Falz) gene (Gene ID 207165) in murine species.
• BPTF, SNF2L and pRBAP46/48 are the three subunits of the nucleosome-remodelling factor NURF, an ISWI chromatin-remodeling complex that catalyzes ATP-dependent nucleosome sliding.
• BPTF mediates NURF target site specificity via interactions with transcription factors, histone variants and histone modifications on transcriptionally active genes.
• BPTF plays an important role in regulating nucleosome occupancy at nucleosome-free regions (NFRs) at sites occupied by the multivalent factors CTCF and cohesin via direct interaction with CTCF and the cohesin subunit SA2.
• In addition, BPTF also interacts with c-MYC and plays an essential role in its genomic distribution and biological activity.
• BPTF is necessary for the survival of c-MYC-overexpressing cells and for c-MYC-driven tumorigenesis in the mouse pancreas.
• BPTF knockdown leads to decreased c-MYC chromatin accessibility and significantly delays tumor development in pre-neoplastic pancreatic acinar cells.
• Consistently, BPTF expression in human tumors positively correlates with activation of c-MYC gene signatures.
• Applications:Suitable for use in Western Blot, Chromatin Immunoprecipitation and Chromatin Immunoprecipitation-seq.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1200 detects BPTF in 10ug of NIH/3T3 cell lysateWestern Blot: 1:1250 detects BPTF in 50ug of wild-type, but not BPTF-knockout, mouse embryonic fibroblast (MEF) lysateOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.