V-type proton ATPase subunit d 2 (UniProt Q80SY3; also known as Osteoclast-specific vacuolar ATP synthase, V-ATPase subunit d 2, Vacuolar proton pump subunit d 2) is encoded by the Atp6v0d2 gene (Gene ID 242341) in murine species.
V-ATPase is a proton pump consists of an 8-subunit (subunit A through H) peripheral catalytic V1 complex (V1) and a 6-subunit (a, c, c , d, e, and c' in yeast or Ac45 in higher eukaryotes) catalytic integral membrane V0 proton pore complex.
V0 subunit d is a 38kD protein located at the cytosolic face of the membrane.
There exist two mammalian subunit d isoforms, d1 and d2, encoded by two separate genes, ATP6V0D1 and ATP6V0D1.
While d1 is expressed ubiquitously, d2 was expressed in specific tissues, such as kidney, lung, and bone.
In addtion, d2 expression level is about 5-fold higher than d1 in osteoclasts.
Atp6v0d1-knockout is embryonic lethal in mice, while Atp6v0d2 deletion does not affect V-ATPase function, but decreases cell to cell fusion.
V-ATPase subunit d 2 transcription is mediated by transcription factor nuclear factor of activated T cells cytoplasmic 1 (NFATc1) that is essential for osteoclastogenesis.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:2000 detects V-ATPase subunit d 2 in mouse osteoclast lysate
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in 0.05% sodium azide.
Specificity：Recognizes mouse V-ATPase subunit d 2. Specificity was confirmed by the lack of cross-reactivity to mouse Atp6v0d1 overexpressed in 293 cells (Lee, S.H., et al. (2006). Nat. Med. 12(12):1403-1409).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo
Immunogen：Linear peptide corresponding to human/mouse/rat V-ATPase subunit d 2 C-terminal end sequence. Species sequence homology: human, non-human primate and rat.
