Aldehyde dehydrogenase family 1 member A3 (EC 1.2.1.5; UniProt Q9JHW9; also known as Aldehyde dehydrogenase 6, RALDH-3, RalDH3, Retinaldehyde dehydrogenase 3) is encoded by the Aldh1a3 (also known as Aldh6, Raldh3) gene (Gene ID 56847) in murine species.
All-trans-retinoic acid (atRA) is a small molecule metabolite of retinol, also known as vitamine A, that plays an important role in the growth and development of many major organ systems, including the postnatal eye growth.
The three retinaldehyde dehydrogenases, RALDH1-3 or ALDH1 1-3, catalyze the irreversible oxidation of retinaldehyde to form atRA.
While retinaldehyde is formed via the reversible oxidation of retinol catalyzed by retinol dehydrogenases (RDHs).
The three RALDH enzymes cooperatively mediate atRA signaling during eye development through tightly regulated spatiotemporal expression patterns.
In the postnatal human eye, RALDH catalytic activity can be detected in the choroid, retinal pigment epithelium (RPE), and retina.
RALDH2 is most abundant in the choroid and RPE, moderate abundance in the retina, and in relatively low abundance in sclera.
RALDH1 is most abundant in the choroid, in moderate abundance in the sclera, and substantially reduced in the retina and RPE.
RALDH3 is most abundantly expressed during embryonic eye development, but not detectable in adult ocular fundal tissues.
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:150 immunostained trabecula analge of the developing eye in paraffin-embedded embryonic E15 Sprague Dawley (SD) rat head sections by fluorescent immunohistochemistryWestern Blot: 1:500 detects RalDH3 in 10ug of human retina tissue lysate
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes mouse RalDH3. Species Crossreactivity: human and rat
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：KLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of mouse RalDH3.
