Adiponectin (UniProt Q8K3R4; also known as 30 kDa adipocyte complement-related protein, ACRP30, Adipocyte complement-related 30 kDa protein, Adipocyte, C1q and collagen domain-containing protein, Adipose most abundant gene transcript 1 protein, apM-1, Gelatin-binding protein) is encoded by the Adipoq (also known as Acdc, Acrp30) gene (Gene ID 246253) in rat.
Adipokines are adipose tissue-secreted factors involved in vascular function and metabolic disease.
While most adipokines are proinflammatory and directly responsible for the development of the chronic inflammatory state in obesity, adiponectin is an adipokine with anti-inflammatory and insulin-sensitizing properties.
Adiponectin mediates it biological activity via adiponection receptor protein 1 & 2 (AdipoR1 & AdipoR2).
Both AdipoR1 and AdipoR2 are reported to be ubiquitously expressed with the highest expression in skeletal muscle and liver, respectively.
Circulating adiponectin levels are reduced in obesity and type II diabetes, whereas weight loss elevates serum adiponectin.
Likewise, adiponectin overexpression is reported to alleviate metabolic dysfunction independent of weight loss in both genetic and diet-induced animal models of obesity.
Aadiponectin deficiency is associated with various clinical cardiovascular disorders including myocardial infarction, peripheral artery disease, and endothelial dysfunction.
Adiponectin is produced with a signal peptide sequence (aa1-18 in human and rat sequence; aa1-17 in mouse), which is removed postranslationally to yield the mature protein with a collagen-like domain (aa42-107 in human and rat, aa45-110 in mouse) and a C1q domain (aa108-244 in human and rat, aa111-247 in mouse).
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:50 detects Adiponectin in rat cardiac muscle and kidney tissue sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes rat Adiponectin. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Mo Rt
Immunogen：Recombinant protein corresponding to rat Adiponectin, fused to GST-Tag.
