WFDC5 encodes a member of the WAP-type four-disulfide core (WFDC) domain family.
Most WFDC proteins contain only one WFDC domain, and this encoded protein contains two WFDC domains.
The WFDC domain, or WAP signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor.
Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric.
The deduced 123aa protein contains an N-terminal signal peptide, followed by 2 WAP domains.
Each WAP domain contains 8 cysteines that form conserved disulfide bridges, and each WAP domain forms a proposed protease-binding loop and a beta sheet.
The mature 99aa WAP1 protein has a calculated molecular mass of 10.9kD.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat WFDC5.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Met1-Gly123 of rat WFDC5, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
