CD179a (VpreB) is a 126 aa-long polypeptide with apparent MW of 16-18kD.
It is expressed selectively at the early stages of B cell development, namely, in proB and early preB cells.
CD179a has an Ig V domain-like structure, but lacks the last beta-strand (beta7) of a typical V domain.
Instead, it has a carboxyl terminal end that shows no sequence homologies to any other proteins.
CD179a associates non-covalently with CD179b (lambda5 or lambda-like) carrying an Ig C domain-like structure to form an Ig light chain-like structure, which is called the surrogate light chain or pseudo light chain.
In this complex, the incomplete V domain of CD179a appears to be complemented by the extra beta7 strand of CD179b.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human VPREB1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Leu33-Met135 of human VPREB1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
