VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages).
VCAM-1 expression is induced by IL-1beta, IL-4, TNF-alpha and IFN-gamma.
VCAM-1 binds to leukocyte integrins VLA-4 and alpha4 beta7.
The rat and mouse SVCAM-1 proteins share approximately 76% aa similarity
VCAM-1 is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium.
This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LDRLEIELLK GETVLENKEF LEDTDMKSLE NKSLEATFIP TTEDTGKALV CQAKLHMDEM EFEPKQRQST QTLYVNVAPR D
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：monkey
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rhesus monkey VCAM1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mk
Immunogen：Recombinant protein corresponding to Leu1-Asp81 of rhesus monkey VCAM1 fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
