VAPB is a type IV membrane protein found in plasma and intracellular vesicle membranes.
The encoded protein is found as a homodimer and as a heterodimer with VAPA.
This protein also can interact with VAMP1 and VAMP2 and may be involved in vesicle trafficking
Sequence analysis predicted that the 243aa VAPB protein, which is 60% homologous to VAPA, contains a conserved N-terminal domain, an alpha-helical coiled-coil domain, and a C-terminal transmembrane domain.
The 99aa VAPC protein is a splice variant of VAPB that retains the N-terminal 70 residues but lacks the coiled-coil and transmembrane domains.
Expression of VAPB was less intense than that of VAPA or the 1.9kb VAPC transcript.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human VAPB.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Pro132 of human VAPB fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
