Ubiquitin, a highly conserved protein involved in the regulation of intracellular protein breakdown, cell cycle regulation, and stress response, is released from degraded proteins by disassembly of the polyubiquitin chains.
During analysis of randomly sampled human coding sequences from the myeloid cell line KG-1, Nomura et al.
(1994) identified a novel cDNA, which they referred to as KIAA0055, that was related to the tre2 oncogene.
Janssen et al.
(1998) found that HUMORF8 encodes a putative deubiquitinating enzyme.
Janssen et al.
(1998) analyzed DNA from a patient with chronic myeloproliferative disorder.
They identified an oncogenic fusion of the 5-prime end of p85-beta with the 3-prime end of HUMORF8.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human USP8.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Thr777-Thr1118 of human USP8, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
