RNTRE is a 97-100kD protein that stably associates in vivo and in vitro with EPS8 via the SH3 domain of the latter.
In vitro, RNTRE displayed remarkable preference for binding to the SH3 domain of EPS8, compared with 8 other SH3s.
A C-terminal truncated mutant of RNTRE was able to confer proliferative advantage and reduced serum requirement to NIH 3T3 fibroblasts, suggesting a role for RNTRE in cell proliferation
Because the 828aa protein encoded by this cDNA shows homology to the N-terminal region of the TRE oncogene, the authors designated it RNTRE, for 'related to the N terminus of TRE.' RNTRE was shown to be ubiquitously expressed.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human USP6NL.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Gly292 of human USP6Nl, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
