URG4 is upregulated in the presence of hepatitis B virus (HBV)-encoded X antigen (HBxAg) and may contribute to the development of hepatocellular carcinoma by promoting hepatocellular growth and survival.In patients infected with HBV, URG4 expression was higher in nontumor tissue than in tumor cells.
URG4 localized to the cytoplasm and, in infected patients, colocalized with HBxAg.
In unaffected individuals, URG4 protein distribution was localized to the cytoplasm in up to 10% of pancreatic ductal epithelial cells and was not detected in any other tissues examined.HepG2 cells overexpressing URG4 displayed increased growth in the absence of HBxAg.
Flow cytometry analysis of URG4 transfected HepG2 cells confirmed that URG4 stimulated cell growth and promoted the entry of cells into S phase.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse URGCP.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Arg689-Arg924 of mouse URGP, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
