Uroplakin 2 is one of the proteins of the highly conserved urothelium-specific integral membrane proteins of the asymmetric unit membrane which forms urothelium apical plaques in mammals.
The asymmetric unit membrane is believed to strengthen the urothelium by preventing cell rupture during bladder distention.
The encoded protein is expressed in the peripheral blood of bladder cancer patients with transitional cell carcinomas
Western blot analysis detected UPK2 at an apparent molecular mass slightly lower than that of 15kD bovine Upk2.
Electron microscopy revealed that AUMs isolated from cattle, dog, mouse, and monkey were 12-nm protein particles forming hexagonally packed crystalline patches.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human UPK2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Asp26-Thr153 of human UPK2, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
