The BUP1 open reading frame of 1,152 bp corresponds to a protein of 384aa with a calculated molecular mass of 43kD, surrounded by a 5-prime untranslated region of 61 nucleotides and a 3-prime untranslated region of 277 nucleotides.
The protein shows 84% identity with the translation product of the rat beta-ureidopropionase cDNA
Expression of the human cDNA in Escherichia coli and eukaryotic COS-7 cells revealed a very high beta-ureidopropionase enzymatic activity, thus confirming the identity of the cDNA.
Human EST libraries from brain, liver, kidney, and heart contained partial beta-ureidopropionase cDNAs, suggesting expression in these tissues, as seen in the rat.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human UPb1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Trp121-Glu384 of human UPb1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
