Human cytomegalovirus (CMV) frequently infects young people and persists for the lifetime of the individual in a latent state, due in part to downregulation of major histocompatibility (MHC) class I expression and evasion of T cell-mediated immunity, with occasional asymptomatic episodes of viral shedding.
ULBP2 has a putative glycosylphosphatidylinositol (GPI) linkage to the membrane.
Enzymatic analysis confirmed that the ULBPs are indeed GPI-linked at the cell surface.
RT-PCR analysis detected ULBP expression in T, B, and erythroleukemia cell lines and in a wide range of tissues; ULBP2, however, was not detected in the Namalwa Burkitt lymphoma cell line.
Expression was upregulated in colon and stomach tumors and downregulated in kidney tumors.
FACS analysis demonstrated that protein expression generally correlated with message expression.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human ULBP2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gly2-Ile246 of human ULBP2, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
