UDP-glucose:glycoprotein glucosyltransferase (UGT) is a soluble protein of the endoplasmic reticulum (ER) that selectively reglucosylates unfolded glycoproteins, thus providing quality control for protein transport out of the ER.
The assembled full-length HUGT1 cDNA encodes a deduced 1555aa protein with a predicted ER-targeting signal sequence, ER retrieval signal, 3 N-glycosylation sites, and 2 disulfide loops.
HUGT1 shares 55% sequence identity with HUGT2 and 31 to 45% identity with Drosophila, C.
elegans, and S.
pombe homologs.
Northern blot analysis detected 3 HUGT1 transcripts of 5.7, 8, and 10.6 kb in pancreas, heart, brain, placenta, lung, liver, skeletal muscle, and kidney.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human UGGT1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Leu1221-Leu1536 of human UGGT1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
