Glycosphingolipids (GSLs) are a group of membrane components that contain lipid and sugar moieties.
They are present in essentially all animal cells and are believed to have important roles in various cellular processes.
UDP-glucose ceramide glucosyltransferase (UGCG) catalyzes the first glycosylation step in GSL biosynthesis.
The product, glucosylceramide, is the core structure of more than 300 GSLs.
Ichikawa et al.
cloned a UGCG cDNA by expressing a human melanoma cell cDNA library in a UGCG-deficient cell line and screening for UGCG complementation.
The predicted 394aa UGCG protein has a potential signal-anchor sequence and a single transmembrane domain near the N terminus, and very hydrophobic regions close to the C terminus, which may interact with the membrane.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human UGCG. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to Lys39-Leu171 of human UGCg, fused to N-terminal His-Tag expressed in E. coli.
