Urocortin-3 is a member of the sauvagine/corticotropin-releasing factor/urotensin I family.
It is structurally related to the corticotropin-releasing factor (CRF) gene and the encoded product is an endogenous ligand for CRF type 2 receptors.
In the brain it may be responsible for the effects of stress on appetite.
In spite of the gene family name similarity, the product of this gene has no sequence similarity to urotensin II.
The mature human SCP peptide sequence shares 72% identity with that of Takifugu rubripes and 57% identity with that of Tetraodon nigroviridis, a freshwater pufferfish.
In human and fish, a stretch of 30 residues at the respective carboxy termini has an extended alpha-helical structure shared by all CRH family peptides.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat UCN3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to His24-Lys164 of rat UCN3, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
