Uridine-cytidine kinase 2 is an enzyme catalyzes the phosphorylation of uridine monophosphate to uridine diphosphate.
This is the first step in the production of the pyrimidine nucleoside triphosphates required for RNA and DNA synthesis.
In addition, an allele of this gene may play a role in mediating nonhumoral immunity to Hemophilus influenzae type B.
Phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate.
Does not phosphorylate deoxyribonucleosides or purine ribonucleosides.
Can use ATP or GTP as a phosphate donor.
Can also phosphorylate cytidine and uridine nucleoside analogs such as 6-azauridine, 5-fluorouridine, 4- thiouridine, 5-bromouridine, N(4)-acetylcytidine, N(4)- benzoylcytidine, 5-fluorocytidine, 2-thiocytidine, 5- methylcytidine, and N(4)-anisoylcytidine.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human UCK2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gly18-His261 of human UCK2, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
