Ubiquitin D is a protein encoded by the UBD gene.Using selective cDNA hybridization of a YAC containing the HLA-F locus region on chromosome 6, followed by Southern and Northern blot analyses, Fan et al.
(1996) isolated a cDNA, which they called 1F12, encoding a protein similar to diubiquitin.
The 1.1-kb transcript was detected only in B-cell lines transformed by Epstein-Barr virus.
By subtractive hybridization of dendritic cell (DC) libraries and EST database searching, Bates et al.
(1997) obtained a full-length cDNA encoding diubiquitin.
The predicted 165aa protein has 2 ubiquitin-like domains, each of which has 2 cysteines.
Southern blot analysis indicated that the cDNA represents a single-copy gene.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat UBD.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Met1-Thr155 of rat UBD, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
