Ubiquitin is a protein encoded by the UBB gene.one of the most conserved proteins known.
Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover.
Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation.
Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression.
This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence.
Consequently, the protein is expressed as a polyubiquitin precursor with a final aa after the last repeat.
Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human Ub. Species Crossreactivity: mouse, rat, rabbit, canine, porcine, equine, and bovine
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Bo Ca Eq Hu Mo Po Rb Rt
Immunogen：Recombinant protein corresponding to Met1-Cys229 of human Ub, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
