Using a human sequence homologous to the bovine UACA sequence to screen a Jurkat cell cDNA library, they isolated the human homolog, which was found have the same sequence as KIAA1561.
Human UACA encodes a deduced 1,449aa protein with a predicted molecular mass of approximately 166kD.
It contains 6 ankyrin repeats, a leucine zipper motif, and coiled-coil domains.
The bovine and human proteins share 86% sequence homology.
Northern blot analysis demonstrated expression at various levels in all tissues analyzed, with highest expression in skeletal muscle.
The prevalence of IgG anti-UACA autoantibodies in patients with panuveitis was significantly higher than that in healthy controls (19.6-28.1% vs 0%, p less than 0.05), indicating that autoimmunity directed against UACA is a common phenomenon in these diseases.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human UACA.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ala29-Glu209 of human UACA, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
