Tryptophan is one of the 20 standard amino acids, as well as an essential amino acid in the human diet.
Only the L-stereoisomer of tryptophan is used in structural or enzyme proteins, but the D-stereoisomer is occasionally found in naturally produced peptides (for example, the marine venom peptide contryphan).
The distinguishing structural characteristic of tryptophan is that it contains an indole functional group.
Plants and microorganisms commonly synthesize tryptophan from shikimic acid or anthranilate.
The latter condenses with phosphoribosylpyrophosphate (PRPP), generating pyrophosphate as a by-product.
After ring opening of the ribose moiety and following reductive decarboxylation, indole-3- glycerinephosphate is produced, which in turn is transformed into indole.
In the last step, tryptophan synthase catalyzes the formation of tryptophan from indole and the amino acid serine.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes Tyr. Species Crossreactivity: pan-species (general)
