TXLN interacts with STX3 through its coiled-coil domain.
TXLN also interacts with STX1A and STX4.
Immunofluorescence and confocal microscopy demonstrated that coexpression of TXLN with STX4 caused relocalization of TXLN from the cytosol to the plasma membrane.
Overexpression of TXLN in PC12 cells inhibited human growth hormone exocytosis in response to high potassium levels. The predicted TXLN protein contains 546aa and has a calculated molecular mass of 62kD.
It has a long C-terminal coiled-coil domain, 24 potential phosphorylation sites, 1 N-glycosylation site, 10 N-myristoylation sites, and 3 EF-hand motifs.
Northern blot analysis detected a major 5.1-kb TXLN transcript in all tissues tested, as well as 2.1- and 1.5-kb TXLN transcripts in heart, liver, and pancreas.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse TXLNa.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Met1-Ala234 of mouse TXLNa, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
