This protein localizes to the centriolar sub-distal appendages that are associated with the older of the two centrioles after centrosome duplication.
This protein plays a central role in organization of the microtubules during centriole duplication.
A pseudogene of this gene is found on chromosome 5
The deduced 475aa protein has a calculated molecular mass of 53kD.
TUBE contains the conserved GTP-binding domain shared with other tubulins, but it lacks the highly acidic C terminus characteristic of alpha-tubulin and beta-tubulin.
Relative to other tubulins, it also has a 26aa insertion between helices 6 and 7.
Localization of TUBE within the centrosome of dividing osteogenic sarcoma cells was dependent on the cell-cycle stage.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human TUBe.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Val225-Ser472 of human TUBe, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
