The deduced 453aa protein has a calculated molecular mass of 51kD.
TUBD1 contains the conserved GTP-binding domain shared with other tubulins, but it lacks the highly acidic C terminus characteristic of alpha-tubulin and beta-tubulin.
Relative to other tubulins, TUBD1 also has a 5aa insertion between helices 6 and 7 and a 21-residue insertion at the end of helix 9
TUBD1 shares 40% aa identity with Chlamydomonas delta-tubulin and 85% identity with mouse delta-tubulin.
Within dividing osteogenic sarcoma cells, TUBD1 partially colocalized with both centrin and gamma-tubulin, but it was most prominent between the centrioles within a centrosome.
Its localization was independent of microtubules.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human TUBd.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Thr190-Leu453 of human TUBd, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
