Protein kinases are generally divided into 2 broad classes based on their ability to phosphorylate either tyrosine (tyr) or serine/threonine (ser/thr) residues.
However, several protein kinases have dual specificity and are able to phosphorylate all 3aa.
TTK encodes a dual specificity protein kinase with the ability to phosphorylate tyrosine, serine and threonine.
Associated with cell proliferation, this protein is essential for chromosome alignment at the centromere during mitosis and is required for centrosome duplication.
It has been found to be a critical mitotic checkpoint protein for accurate segregation of chromosomes during mitosis.
Tumorigenesis may occur when this protein fails to degrade and produces excess centrosomes resulting in aberrant mitotic spindles.
Alternative splicing results in multiple transcript variants.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human TTK.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gly532-Leu786 of human TTK, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
