Transcription termination by RNA polymerase I is largely the result of binding of a transcription termination factor to a DNA element called a transcriptional terminator.
The terminator element is located downstream of the pre-rRNA coding region (RNR1) and in many species is also found immediately upstream of the gene promoters for the tandemly repeated rRNA genes.
The mouse protein was partially purified and limited peptide sequence was obtained.
The resulting cDNA encoded a predicted 833aa protein.
Recombinant mouse TTF1 specifically bound to the mouse terminator elements and stopped transcription in an in vitro system.
Study of the protein identified a large C-terminal DNA-binding domain, with sequence similarity to the c-Myb oncogene, and an internal domain required for transcription termination.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human TTF1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Gly204 of human TTF1, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
