Tsk is a BMP inhibitor in oviparous vertebrates that forms a ternary complex with Bmp and chordin and is involved in the regulation of primitive streak and Hensen node
Using transcript-specific PCR primers for novel sequences that increased more than 10-fold upon treatment with 17-beta estradiol (E2), they cloned 5 cDNAs, designated E2-induced genes (E2IG) 1-5, from a human placenta cDNA library.
The E2IG4 cDNA encodes a deduced 353aa protein containing a leucine-rich repeat in the N terminus and 8 additional leucine-rich repeats.
E2IG4 demonstrates domain and structural homology to extracellular matrix leucine-rich proteoglycans such as decorin and biglycan.
It also contains various potential phosphorylation sites.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse TSK.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Glu30-Gln307 of mouse TSK, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
