The thyrotropin receptor is a protein responds to thyroid-stimulating hormone (also known as "thyrotropin", and stimulates the production of thyroxine (T4) and triiodothyronine (T3).
The TSH receptor is a member of the G-protein coupled receptor superfamily of integral membrane proteins.It is primarily found on the surface of the thyroid epithelial cells.It should not be confused with "thyrotropin-releasing hormone" (TRH) or "thyrotropin-releasing hormone receptor" (TRHR).
The deduced 764aa protein has a molecular mass of 86.8kD and contains a signal peptide, 7 transmembrane regions, 5 potential glycosylation sites, and a short intracytoplasmic region.
The TSHR cDNA encoded a functional receptor that activated adenylate cyclase in response to TSH.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat TSHR.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Pro28-Gly245of rat TSHR, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
