Thyrotropin-stimulating hormone (TSH) is a noncovalently linked glycoprotein heterodimer and is part of a family of pituitary hormones containing a common alpha subunit (TSHA) and a unique beta subunit that confers specificity.Using bovine TSHB cDNA as probe, Hayashizaki et al.
(1985) cloned TSHB from human liver and leukocyte genomic DNA libraries.
Human TSHB encodes a deduced protein consisting of a 20aa signal sequence, a mature protein of 112aa, and a C-terminal extension of 6 hydrophobic aa.
Wondisford et al.
(1988) also cloned the human TSHB gene.Wondisford et al.
(1988) determined that the TSHB gene contains 3 exons, the first of which is noncoding.
The rat TSHB gene also contains one 5-prime noncoding exon, whereas the mouse Tshb gene contains 3.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：canine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes canine TSHb.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Ca
Immunogen：Recombinant protein corresponding to Ser20-Ile138 of canine TSHb, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
