TRRAP is an adapter protein, which is found in various multiprotein chromatin complexes with histone acetyltransferase activity (HAT), which in turn is responsible for epigenetic transcription activation.
TRRAP a central role in MYC (c-Myc) transcription activation, and also participates in cell transformation by MYC
TRRAP is also required for the mitotic checkpoint and normal cell cycle progression.
The MRN complex (composed of MRE11, RAD50 (gene), and NBS1) is involved in the detection and repair of DNA double-strand breaks (DSBs).
TRRAP associates with the MRN complex and when TRRAP is removed, the complex shows reduced cDNA end-joining activity.
Hence TRRAP may function as a link between DSB repair and chromatin remodeling.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human TRRAP.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ala3534-Leu3770 of human TRRAP, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
