TRPV6 is a member of the Transient receptor potential (TRP) family of membrane proteins.
Unlike most TRP channels, TRPV6 is selective for Ca2+ ions, similarly to its close homologue, TRPV5, which is mainly expressed in the kidney, and plays a role in Ca2+ reabsorption.
Expression of TRPV6 appears to be vitamin D dependent as it is greatly reduced in animals that do not express the vitamin D receptor.
Importantly, vitamin D treatment of colon cancer cells, Caco-2, also increases expression of TRPV6 gene.
Vitamin D treatment of these cells also increases transport of calcium, probably via increasing TRPV6 expression.The TRPV6 calcium transporter also found in the human placenta, pancreas and prostate gland and in some species in the kidney where the related channel TRPV5 is strongly expressed.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human TRPV6.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met578-Ile725 of human TRPV6, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
