TRP-PLIK is a protein that is both an ion channel and a kinase.
As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium.
As a kinase, it is capable of phosphorylating itself and other substrates.
The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants.The interaction of PLIK with PLCB1 was confirmed by coimmunoprecipitation of expressed proteins in CHO-K1 cells.
Northern blot analysis revealed an approximately 8-kb transcript in brain and skeletal muscle, with stronger signals in kidney, heart, liver, and spleen, consistent with the 7,105-bp transcript size for TRP-PLIK.
A hemagglutinin-tagged TRP-PLIK protein was found to be membrane bound.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat TRPM7.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Ile19-Gly179 of rat TRPM7, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
