TRIP6 is a member of the zyxin family and encodes a protein with three LIM zinc-binding domains.
This protein localizes to focal adhesion sites and along actin stress fibers.
Recruitment of this protein to the plasma membrane occurs in a lysophosphatidic acid dependent manner and it regulates LPA-induced cell migration.
Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.Deconvolution microscopy demonstrated diffuse cytoplasmic colocalization of srfH and Trip6 in murine macrophages.
Bacteria lacking SrfH were unable to stimulate TRIP6-dependent phagocyte migration in vitro or in vivo.
Mice lacking Cd18 and infected with Salmonella did not show phagocyte migration, in spite of diminished bactericidal function.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human TRIP6.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Cys279-Cys476 of human TRIP6, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
