Transcriptional-regulating factor 1 is a zinc-finger transcriptional regulating protein which interacts with CBP/p300 to regulate the human gene CYP11A1.The deduced 1,200aa protein contains a glutamine-rich region, 2 proline-rich regions, an acidic region, and 3 C2H2-type zinc finger motifs.
An alternatively spliced transcript with a longer 3-prime UTR encodes the same protein.
Northern blot analysis detected transcripts of 4.4 and 7.5 kb abundantly expressed in thymus, testis, adrenal cortex, and a human choriocarcinoma cell line.
Weak expression was detected in all other tissues examined except pancreas and small intestine.
In vitro transcription and translation resulted in a protein with an apparent molecular mass of about 132kD, in agreement with the predicted size.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human TRERF1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Pro779-Arg936 of human TRERF1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
