84-4440-51　TREH (Trehalase, TRE, TREA, Brush Border Membrane Glycoprotein, Alpha,Alpha-Trehalase, Alpha,Alpha-Trehalose Glucohydrolase) 200ul　365434

特徴

• Trehalose (1-alpha-D-glucopyranosyl alpha-D-glucopyranoside) is widely distributed in nature, being the storage disaccharide of fungi and the blood sugar of insects.
• Trehalase (EC 3.2.1.28) is an intrinsic glycoprotein of the small intestine and renal brush border membranes that hydrolyzes trehalose to 2 glucose molecules.
• The deduced 583aa protein has a calculated molecular mass of 66.6kD.
• It has an N-terminal signal peptide, 5 potential N-glycosylation sites, and a C-terminal hydrophobic region for glycosylphosphatidylinositol (GPI) attachment.
• Northern blot analysis detected a 2.0-kb transcript mainly in kidney, liver, and small intestine.The trehalase signature sequence from aa162 to 175 of mouse Treh shares 100% identity with the corresponding region in human TREH.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- SLIYSKHPFI VPGGRFVEFY YWDSYWVMEG LLLSEMASTV KGMLQNFLDL VKTYGHIPNG GRIYYLQRSQ PPLLTLMMDR YVAHTKDVAF LQENIGTLAS ELDFWTVNRT VSVVSGGQSY VLNRYYVPYG GPRPESYRKD AELANSVPEG DRETLWAELK AGAESGWDFS SRWLVGGPDP DLLSSIRTSK MVPADLNAFL CQAEELMSNF YSRLGNDTEA TKYRNLRAQR LAAMEAVLWD EQKGAWFDYD LEKGKKNLEF Y
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.