TRAIL is a recently identified member of the TNF gene superfamily.
Five different receptors have been identified for TRAIL.
Two receptors, DR4 and DR5, are transmembrane proteins containing death domain similar to FAS and other TNF family receptors.
Two other receptors, DcR1 and DcR2, act like decoy proteins for TRAIL binding because they lack the death domain.
TRAIL can also bind, though weakly, to osteoprotegrin (OPG), a soluble receptor, which plays a role in osteoclastogenesis.
TRAIL induces apoptosis in various tumor cell lines, whereas most primary cells seem to be resistant.
TRAIL-mediated apoptosis occurs following its binding to DR4 or DR5 receptors.
The mechanism of apoptosis involves activation of caspase-8 and subsequent activation of effector caspases.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：canine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes canine TRAIL.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Ca
Immunogen：Recombinant protein corresponding to Thr36-Gly281 of canine TRIL, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
