84-4440-37　TPO (Thrombopoietin, THPO, MGDF, MKCSF, ML, MPLLG, Myeloproliferative Leukemia Virus Oncogene Ligand, Megakaryocyte Growth And Development Factor, Megakaryocyte Stimulating Factor) 200ul　365420

特徴

• Thrombopoietin (TPO), is a key regulator of megakaryocytopoiesis and thrombopoiesis in vitroand in vivo.
• TPO has been purified and cloned from several species including mouse, rat, and dog.
• The proteins from the various species are highly conserved, exhibiting from 69-75% sequence identity at the amino acid sequence level.
• Mature TPO can be divided into two domains: the amino-terminal half with homology to erythropoietin (Epo) and the carboxy-terminal half rich in serine, threonine and proline residues and containing seven potential N-linked glycosylation sites.
• The carboxy terminus domain of TPO has been shown to regulate the specific activity and circulating half-life of TPO.
• The carboxy-terminal may also have a role in promoting the efficient biosynthesis and secretion of TPO.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-VPPACDP RLLNKLLRDS YLLHSRLSQC PDVNPLSIPV LLPAVDFSLG EWKTQTEQSK AQDILGAVSL LLEGVMAARG QLEPSCLSSL LGQLSGQVRL LLGALQGLLG TQLPPQGRTT AHKDPSALFL SLQQLLRGKV RFLLLVEGPA LCVRRTLPTT AVPSRTSQLL TLNKFPNRTS GLLETNFSVV ARTAGPGLLN RLQGFRAKII PGQLNQTSGS LDQIPGYLNG THEPVNGTHG LFAGTS
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.