Thrombopoietin (TPO), is a key regulator of megakaryocytopoiesis and thrombopoiesis in vitroand in vivo.
TPO has been purified and cloned from several species including mouse, rat, and dog.
The proteins from the various species are highly conserved, exhibiting from 69-75% sequence identity at the amino acid sequence level.
Mature TPO can be divided into two domains: the amino-terminal half with homology to erythropoietin (Epo) and the carboxy-terminal half rich in serine, threonine and proline residues and containing seven potential N-linked glycosylation sites.
The carboxy terminus domain of TPO has been shown to regulate the specific activity and circulating half-life of TPO.
The carboxy-terminal may also have a role in promoting the efficient biosynthesis and secretion of TPO.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human TPO.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Pro25-His298 of human TPO, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
