Tissue plasminogen activator is a protein involved in the breakdown of blood clots.
Specifically, it is a serine protease (EC 3.4.21.68) found on endothelial cells, the cells that line the blood vessels.
As an enzyme, it catalyzes the conversion of plasminogen to plasmin, the major enzyme responsible for clot breakdown.
Because it works on the clotting system, tPA is used in clinical medicine to treat only embolic or thrombolytic stroke.
Use is contraindicated in hemorrhagic stroke and head trauma.The classic role of tPA is in the clotting system.
Specifically, tPA catalyzes the conversion of plasminogen into plasmin.
It does so by cleaving the single-chained plasminogen into two chains.
These two chains are linked by a disulfide bond and the resulting molecule is called plasmin.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat tPA.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Gly311-Asn487 of rat tPA, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
