Platelet-derived endothelial cell growth factor is a novel angiogenic factor distinct from the previously described endothelial cell mitogens of the fibroblast growth factor family.
PD-ECGF, which is stored in platelets as a 45kD single polypeptide chain, has a highly restricted target cell specificity acting only on endothelial cells.
It promotes angiogenesis in vivo and stimulates the in vitro growth of a variety of endothelial cells.
Cloning of the gene revealed that it contains 10 exons spanning more than 4.3 kb.
Using an ECGF1 cDNA probe, Stenman et al.
assigned the ECGF1 gene to chromosome 22 by analysis of its segregation in a panel of human/rodent somatic cell hybrids.
By in situ hybridization, they sublocalized the gene to 22q13.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2ug/mlImmunohistochemistry (Paraffin): 5-30ug/mlImmunocytochemistry: 5-30ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse TP.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Lys29-Gln305 of mouse TP, expressed in E. coli.
