By exon trapping on chromosome 9q34, screening fetal brain, adult frontal cortex, and adult liver cDNA libraries, and 5-prime and 3-prime RACE, Ozelius et al.
(1997) cloned TOR1B, which they designated DQ1.
The deduced 289aa protein has a calculated molecular mass of more than 32kD.
TOR1B shares 69% amino acid identity with TOR1A, and both proteins contain an ATP-binding domain, 2 protein kinase C phosphorylation sites, a casein kinase II phosphorylation site, several N-myristoylation sites, and 6 conserved cysteines.
Northern blot analysis detected expression of a 2.8-kb TOR1B transcript in adult brain, but not in fetal brain.TOR1B lies centromeric to TOR1A, and the 2 genes face in opposite orientations with their 3-prime ends about 2 kb apart.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse TOR1B.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Thr83-Lys327 of mouse TOR1B, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
