84-4440-11　［受注停止］TOP1 (Topoisomerase I, TOPI, DNA Topoisomerase 1) 200ul　365394

特徴

• DNA topoisomerase 1 isa DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription.
• This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA.
• This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.Type IA topoisomerases creates a single break in DNA, and passes a second strand or duplex through the break.
• This strand passage mechanism shares several features with type IIA topoisomerases.
• They both form a 5' phosphotyrosine intermediate, and require a divalent metal ion to perform its work.
• Unlike type II topoisomerases, type IA topoisomerases do not use energy to do its work .
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MSGDHLHNDS QIEADFRLND SHKHKDKHKD REHRHKEHKK EKDREKSKHS NSEHKDSEKK HKEKEKTKHK DGSSEKHKDK HKDRDKEKRK EEKVRASGDA KIKKEKENGF SSPPQIKDEP EDDGYFVPPK EDIKPLKRPR DEDDADYKPK KIKTEDTKKE KKRKLEEEED GKLKKPKNKD KDKKVPEPDN KKKKPKKEEE QKWKWWEEER YPEGIKWKFL EHKGPVFAPP YEPLPENVKF YYDGKVMKLS
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.