Tensin-3 formed a complex with focal adhesion kinase and p130Cas (BCAR1) in human breast carcinoma cells.
Addition of EGF to these cells induced dephosphorylation of FAK and p130Cas, leading to dissociation of the tensin-3/FAK/p130Cas complex and enhanced interaction between tensin-3 and EGFR.
The deduced 1445aa protein has a calculated molecular mass of 155.3kD.
Like other tensins, tensin-3 has an N-terminal actin-binding domain and C-terminal SH2 and PTB domains.
It also has several potential tyrosine phosphorylation sites.
Northern blot analysis detected high expression of an 8-kb transcript in kidney and placenta, with weaker expression in heart, skeletal muscle, spleen, liver, and lung.
Western blot analysis revealed a 180kD tensin-3 protein in human endothelial and epithelial cells and fibroblasts.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human TNS3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Thr301 of human TNS3, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
