Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction.
It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC).
Each subunit is responsible for part of troponin complex function.
TnI inhibits ATPase activity of acto myosin and TnT and TnI are present in cardiac muscles in different forms than in skeletal muscles.
Only one tissue specific isoform of TnI is described for cardiac muscle tissue (cTnI) and this is expressed only in myocardium.Equally reactive with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components.
(In the presence of 5 mM EDTA).
Antibodies are not affected by heparin, phosphorylation, oxidation and troponin complex formation.
No cross-reaction with skeletal muscle troponin I.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：porcine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes porcine TNNI3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Po
Immunogen：Recombinant protein corresponding to Ala2-Gly211 of porcine TNNI3, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
